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anti γh2ax antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti γh2ax antibody
    Anti γh2ax Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 3730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B3h2ax/Phospho-Histone+H2A%2EX+(Ser139)+Rabbit+mAb/pm42871965-182-34-36
    Average 99 stars, based on 3730 article reviews
    anti γh2ax antibody - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: HPV16 recruitment of SMARCAL1 to viral and host replication forks is required for the viral life cycle.
    Article Snippet: PLA was performed using the Duolink In Situ Red Starter Kit (Sigma) following the manufacturer’s protocol. .. Fixed cells were incubated with primary antibodies against 53BP1 (Invitrogen, PA1-16565) and γH2AX (Cell Signaling Technology, 9718), SMARCAL1 (1:1,000, Abcam, ab154226), RPA34-20 (1:1,000, (Merck). ..

    Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
    Article Snippet: Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions. .. After blocking nonspecific sites with 5% non-fat dry milk in TBST for 1 h and washing with TBST, the membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies (1:1000) against γH2AX (cat. no.: 9718), and caspase-3 (cat. no.: D3R6Y), from Cell Signaling Technology, Inc. (Danvers, MA, USA), and mouse monoclonal anti-β-actin antibody (cat. no. A1878, Merck Life Science (Poznań, Poland)), followed by incubation with anti-rabbit IgG horseradish peroxidase-conjugated (cat. no.: 7074, Cell Signaling Technology) or anti-mouse IgG HRP-conjugated (cat. no.: A28177 , Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) secondary antibodies for 1 h at room temperature, . .. To obtain a chemiluminescence signal, an enhanced chemiluminescent (ECL) substrate was used (SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA)).

    Blocking Assay:

    Article Title: Proton FLASH Exposure Preserves Gut Commensal Microbiomes and Spares Intestinal Stem Cells.
    Article Snippet: .. After antigen etrieval, sections were blocked with a peroxidase block and ncubated with the primary antibody anti-Ki67 (Cell Signaling, at No. 12202, 1:1500 dilution) and γH2AX (Cell Signaling, Cat o. .. 9718, 1:1000 dilution), ant-CD31 (Cell Signaling, Cat No. 77699, :250 dilution), overnight at 4◦C in t dark humidified chamber. ollowed by detected with an HRP-conjugated goat-anti-rabbit econdary antibody.

    Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
    Article Snippet: Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions. .. After blocking nonspecific sites with 5% non-fat dry milk in TBST for 1 h and washing with TBST, the membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies (1:1000) against γH2AX (cat. no.: 9718), and caspase-3 (cat. no.: D3R6Y), from Cell Signaling Technology, Inc. (Danvers, MA, USA), and mouse monoclonal anti-β-actin antibody (cat. no. A1878, Merck Life Science (Poznań, Poland)), followed by incubation with anti-rabbit IgG horseradish peroxidase-conjugated (cat. no.: 7074, Cell Signaling Technology) or anti-mouse IgG HRP-conjugated (cat. no.: A28177 , Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) secondary antibodies for 1 h at room temperature, . .. To obtain a chemiluminescence signal, an enhanced chemiluminescent (ECL) substrate was used (SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA)).

    Bioprocessing:

    Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
    Article Snippet: Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions. .. After blocking nonspecific sites with 5% non-fat dry milk in TBST for 1 h and washing with TBST, the membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies (1:1000) against γH2AX (cat. no.: 9718), and caspase-3 (cat. no.: D3R6Y), from Cell Signaling Technology, Inc. (Danvers, MA, USA), and mouse monoclonal anti-β-actin antibody (cat. no. A1878, Merck Life Science (Poznań, Poland)), followed by incubation with anti-rabbit IgG horseradish peroxidase-conjugated (cat. no.: 7074, Cell Signaling Technology) or anti-mouse IgG HRP-conjugated (cat. no.: A28177 , Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) secondary antibodies for 1 h at room temperature, . .. To obtain a chemiluminescence signal, an enhanced chemiluminescent (ECL) substrate was used (SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA)).

    Immunofluorescence:

    Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1
    Article Snippet: Western blot images were acquired using SYNGENE PXi instrument. .. Immunofluorescence was performed to analyze STING localization and activation (STING 1:500-Cell Signaling- 13647, S366 pSTING 1:1200- Cell Signaling-50907), BrdU quantification (1:200- Santa Cruz -sc20045), levels of S33 p-RPA (1:1000- Bethyl -PLA0070), γH2AX (1:200 Cell Signalling-2577) and 53BP1 (1:1000- Santa Cruz- sc517281). ..

    Activation Assay:

    Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1
    Article Snippet: Western blot images were acquired using SYNGENE PXi instrument. .. Immunofluorescence was performed to analyze STING localization and activation (STING 1:500-Cell Signaling- 13647, S366 pSTING 1:1200- Cell Signaling-50907), BrdU quantification (1:200- Santa Cruz -sc20045), levels of S33 p-RPA (1:1000- Bethyl -PLA0070), γH2AX (1:200 Cell Signalling-2577) and 53BP1 (1:1000- Santa Cruz- sc517281). ..

    other:

    Article Title: MOB2 Loss Sensitizes Lung Cancer Cells to PARP Inhibition Through p53-Dependent DNA Damage Signaling.
    Article Snippet: Densitometric analysis was performed using ImageJ (version 1.54, NIH, Bethesda, MD, USA) software, with protein levels normalized to the corresponding loading controls.



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    Cell Signaling Technology Inc γh2ax antibody
    Inhibition of ATM restores NSCLC cells to IFN-γ by inducing DNA damage response (A) Cell viability of A549 (left panel) or PC-9 (right panel) treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05. (B) Expression of <t>γH2AX</t> and b-Actin (loading control) in A549 (left panel) or PC-9 (right panel) cells treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown.
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    Image Search Results


    Journal: Advanced Science

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

    doi: 10.1002/advs.78290

    Figure Lengend Snippet:

    Article Snippet: γH2AX (Ser139) , 9718 , Cell Signaling Technology (CST) , AB_2118009.

    Techniques: Cell-Signaling

    Inhibition of ATM restores NSCLC cells to IFN-γ by inducing DNA damage response (A) Cell viability of A549 (left panel) or PC-9 (right panel) treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05. (B) Expression of γH2AX and b-Actin (loading control) in A549 (left panel) or PC-9 (right panel) cells treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown.

    Journal: Biochemistry and Biophysics Reports

    Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

    doi: 10.1016/j.bbrep.2026.102568

    Figure Lengend Snippet: Inhibition of ATM restores NSCLC cells to IFN-γ by inducing DNA damage response (A) Cell viability of A549 (left panel) or PC-9 (right panel) treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05. (B) Expression of γH2AX and b-Actin (loading control) in A549 (left panel) or PC-9 (right panel) cells treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown.

    Article Snippet: Ferrostatin-1 and Liproxstatin-1 were purchased from Cayman, and the GSH/GSSG Quantification Kit and Cell Counting WST-8 were purchased from Dojindo. γH2AX antibody and β-Actin were purchased from Cell Signaling Technology.

    Techniques: Inhibition, Expressing, Control