anti γh2ax antibody (Cell Signaling Technology Inc)
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Anti γh2ax Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 3730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B3h2ax/Phospho-Histone+H2A%2EX+(Ser139)+Rabbit+mAb/pm42871965-182-34-36
Average 99 stars, based on 3730 article reviews
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Incubation:Article Title: HPV16 recruitment of SMARCAL1 to viral and host replication forks is required for the viral life cycle. Article Snippet: PLA was performed using the Duolink In Situ Red Starter Kit (Sigma) following the manufacturer’s protocol. .. Fixed cells were incubated with primary antibodies against 53BP1 (Invitrogen, PA1-16565) and Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer Article Snippet: Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions. .. After blocking nonspecific sites with 5% non-fat dry milk in TBST for 1 h and washing with TBST, the membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies (1:1000) against Blocking Assay:Article Title: Proton FLASH Exposure Preserves Gut Commensal Microbiomes and Spares Intestinal Stem Cells. Article Snippet: .. After antigen etrieval, sections were blocked with a peroxidase block and ncubated with the primary antibody anti-Ki67 (Cell Signaling, at No. 12202, 1:1500 dilution) and Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer Article Snippet: Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions. .. After blocking nonspecific sites with 5% non-fat dry milk in TBST for 1 h and washing with TBST, the membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies (1:1000) against Bioprocessing:Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer Article Snippet: Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions. .. After blocking nonspecific sites with 5% non-fat dry milk in TBST for 1 h and washing with TBST, the membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies (1:1000) against Immunofluorescence:Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1 Article Snippet: Western blot images were acquired using SYNGENE PXi instrument. .. Immunofluorescence was performed to analyze STING localization and activation (STING 1:500-Cell Signaling- 13647, S366 pSTING 1:1200- Cell Signaling-50907), BrdU quantification (1:200- Santa Cruz -sc20045), levels of S33 p-RPA (1:1000- Bethyl -PLA0070), Activation Assay:Article Title: STING causes replication stress and nascent DNA degradation via SAMHD1 Article Snippet: Western blot images were acquired using SYNGENE PXi instrument. .. Immunofluorescence was performed to analyze STING localization and activation (STING 1:500-Cell Signaling- 13647, S366 pSTING 1:1200- Cell Signaling-50907), BrdU quantification (1:200- Santa Cruz -sc20045), levels of S33 p-RPA (1:1000- Bethyl -PLA0070), other:Article Title: MOB2 Loss Sensitizes Lung Cancer Cells to PARP Inhibition Through p53-Dependent DNA Damage Signaling. Article Snippet: Densitometric analysis was performed using ImageJ (version 1.54, NIH, Bethesda, MD, USA) software, with protein levels normalized to the corresponding loading controls. |

